ABSTRACT
BACKGROUND: A critical shortage of donor organs has necessitated an investigation of new strategies to increase the availability of additional organs available for human transplantation. We investigated the amount of apoptosis and expression of GADD45beta in two groups, a GADD45beta-transfected group and untransfected group. MATERIAL AND METHOD: The experimental groups consist of a control group (normal H9C2 cell line) and GADD45beta-transfected group. After injury of the each group, we evaluated the expression of GADD45beta and the level of apoptosis in each group. RESULT: There was a significant increase in the expression of GADD45beta in the GADD45beta-transfected group at 1 hour, 2 hours, and 3 hours after stimuli as compared with the control group. The amount of cardiac myoblast cell line apoptosis was significantly lower in the GADD45beta-transfected group as compared with the control group. The concentration of annexin in the GADD45beta-transfected group was significantly lower than that of the control group after cell injury. CONCLUSION: Transfection of a rat myoblast cell line with the GADD45beta gene results in decreased susceptibility to cell injury of human serum.
Subject(s)
Animals , Humans , Rats , Apoptosis , Cell Line , Myoblasts , Myoblasts, Cardiac , Tissue Donors , Transfection , Transplantation, Heterologous , TransplantsABSTRACT
Objective To investigate the expression of Gadd45beta protein in human hepatocellular carcinoma(HCC) and its clinicopathologic significance.Methods Immunohistochemical method was used to detect the expressions of Gadd45beta in both hepatocellular carcinoma tissues and adjacent non-neoplastic liver tissues form 50 cases.Results The positive rates of Gadd45beta expression in the hepatocellular carcinoma tissues and adjacent non-neoplastic liver tissues were 26.0%(13/50) and 82.0%(41/50) respectively.There were significant difference for the positive rate between the two groups(P0.05).Conclusion Low expression of Gadd45beta protein in hepatocellular carcinoma tissues was exhibited,which may be related to hepatocarcinogenesis as well as biological behaviors and prognosis of HCC.